Functional fungal pellets self-immobilized by mycelium fragments of Irpex lacteus WRF-IL for efficient degradation of sulfamethazine as the sole carbon source.

In order to achieve an efficient microbial material with dual functions of self-immobilization and sulfamethazine (SMZ) degradation, this study explored the pelletization technique utilizing mycelium fragments of Irpex lacteus WRF-IL and systematically examined the pellets formation conditions and degradation capability. The Box-Behnken design results demonstrated that pure mycelium fragments, broken by frosted glass beads, could be rapidly self-immobilized to form white rot mycelial pellets (WRMPs) within 24 h, serving as the pelleting core. These WRMPs could completely remove SMZ as the sole carbon source within 20 h. The addition of sucrose expedited this process, achieving complete removal within only 14 h. Kinetic analysis showed that WRMPs could potentially remove SMZ at higher concentrations (>25 mg/L). Biodegradation was the primary pathway of SMZ removal. Seven intermediates were identified by QTOF LC/MS, and three transformation pathways initiated by SO2 overflow, molecular rearrangement, and aniline moiety oxidation were deduced.

Self-assembly biochar colloids mycelial pellet for heavy metal removal from aqueous solution.

To effectively improve the heavy metal removal efficiency and stability of biomass adsorbents, a novel biochar colloids-mycelial pellets (BC-MP) composite was prepared via a biological assembly method. BC-MP was successfully produced with increased surface area and multisorption sites by physical adsorption, electrostatic interaction and hydrogen-bond formation between BC and extracellular polymers on MP. To investigate the performance and mechanisms of heavy metal adsorption by BC-MP, batch experiments were conducted with cadmium (Cd (II)) as the model pollutant. Results showed that BC-MP had higher removal efficiency (57.66%) compared to BC (5.45%) and MP (38.45%), respectively, due to the synergistic effect. The maximum adsorption capacity of Cd (II) on BC-MP was 102.04 mg/g based on Langmuir isotherm model. Adsorption kinetics analysis indicated that chemical sorption was the key factor controlling the adsorption of Cd (II) onto BC-MP. Multiple characterization tests revealed that the main mechanisms of the adsorption process were surface complexation, cation exchange and precipitation. The BC-MP composite showed excellent heavy metal removal efficiency with long-term adsorption stability, suggesting its potential as a promising biosorbent for heavy metal removal from industrial wastewater.

A bio-functions integration microcosm: Self-immobilized biochar-pellets combined with two strains of bacteria to remove atrazine in water and mechanisms.

A self-immobilization method for microorganisms was developed based on fungal pellets. Generally, pellets have some problems such as cell leakage, cell loading limitation and low mechanical strength. Therefore, biochar was applied to overcome these disadvantages. Atrazine degradable microorganism Arthrobacter sp. ZXY-2 was immobilized by Aspergillus niger Y3 pellets. After adding biochar with optimal dosage (0.006 g biochar for 0.3 g pellets with ZXY-2), the self-immobilized biomixture (SIB) removed 50 mg /L atrazine rapidly within 1 h, which was 61% higher compared to pellets without biochar. The kinetic adsorption results showed that the biosorption of biochar by pellets followed a pseudo-second-order kinetic model. The ATZ removal ability and reusability of SIB were significantly increased by biochar. The results showed that the addition of biochar could enhance the connection between ZXY-2 and pellets based carrier, and the favorable biodegradation pH of ZXY-2 changed to 6 and 10. Several analyses such as ζ-potential measurements, FTIR, XPS, SEM-EDS, and elemental analyses were performed to evaluate the mechanism of action of SIB. To enhance the ATZ degradation by single strain, Agrobacterium, sp WL-1 was isolated and added. The metabolic pathways and their function complementation were studied. Furthermore, a biomass integration model for wastewater treatment was proposed herein.

Self-immobilized biomixture with pellets of Aspergillus niger Y3 and Arthrobacter. sp ZXY-2 to remove atrazine in water: A bio-functions integration system.

The microorganism Arthrobacter. ZXY-2 exhibits excellent degradation efficiency for atrazine in free cells. However, its poor fixability makes it hard to be kept and recycled in water. To conquer the problem, this work employed mycelial pellets of Aspergillus niger Y3 to immobilize ZXY-2, which formed a self-immobilized biomixture (SIB) to remove atrazine. SIB could completely degrade 57.3 mg/L atrazine within 10 h. The SIB exhibited the highest degradation efficiency at pH = 7 and 40 °C. Degradation of atrazine with initial concentrations of 57.3 mg/L and 17.5 mg/L was described well by zero and first-order reaction kinetics, respectively. The recycling experiments demonstrated that SIB could be recycled for 5 batches. The results of SEM, FT-IR, and zeta potential analysis showed that porous structure, functional groups, and electronegativity of SIB all contributed to its stable formation. Therefore, this study demonstrates that SIB could be formed stably and could remove atrazine efficiently.

Novel self-immobilized biomass mixture based on mycelium pellets for wastewater treatment: A review.

Mycelial pellets, as a novel biomass material, can adsorb pollutants as a biosorbent, or combine other substances and organisms to form self-immobilized biomixture (SIB) to remove pollutants from wastewater. The pellets are eco-friendly, have a good self-immobilization capacity, and are easy to filter. In addition, some mycelial fungi can remove the pollutants in water through biodegradation. This study reviewed biomixture based on mycelial pellets and the two ways, through which SIB remove pollutants in water: pure pellets and the pellets with other materials. The characteristics and functions of each part of SIB were discussed. The study also highlighted the shortcomings of the technology and provided recommendations for further development of this technology.